7/11/2008 (15h00 - ONERA - 29, avenue de la division Leclerc - 92320 Châtillon - Salle de réunion P. Malavard)
Boston University/Department of Biomedical Engineering
New approaches toward fluorescence background rejection

Résumé : I will describe a new method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low resolution. This is complemented with highresolution information obtained from the uniform-illumination image. A fusion of both images leads to a full resolution image that is optically sectioned across all spatial frequencies. This hybrid illumination method is fast, robust, and provides a user-defined depth of field. Moreover it is generalizable to a variety of illumination and imaging configurations. Demonstrations will be presented of brain tissue imaging with a standard microscope and colon tissue imaging with an endomicroscope.

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